Journal: Oncology Letters

Article Title: Synchronous liver metastasis at initial diagnosis of adrenal pheochromocytoma by CT: A case report

doi: 10.3892/ol.2024.14462

Figure Lengend Snippet: Postoperative pathological analysis. (A) HE staining results of adrenal mass. (B) HE staining results of liver mass. (C-E) Immunohistochemistry of liver mass: (C) Chromogranin A (+) and synaptophysin (+); (D) α-fetoprotein (−); and (E) cytokeratin (−) (magnification, ×400). Blue staining indicates negative expression, whereas brown staining indicates positive expression.

Article Snippet: The antibodies against chromogranin A (cat. no. #ZM-0076; dilution 1:200), synaptophysin (cat. no. #ZM-0246; dilution 1:200), CK7 (cat. no. #ZA-0573; dilution 1:200), CK20 (cat. no. #ZA-0574; dilution 1:200), α-fetoprotein (cat. no. #ZM-0009; dilution 1:200), and α-inhibin (cat. no. #ZM-0460; dilution 1:200) were from Origene Technologies, Inc.

Techniques: Staining, Immunohistochemistry, Expressing

Journal: Brain, behavior, and immunity

Article Title: Rats bred for low intrinsic aerobic exercise capacity link obesity with brain inflammation and reduced structural plasticity of the hippocampus.

doi: 10.1016/j.bbi.2021.06.017

Figure Lengend Snippet: Fig. 4. Older rats expressed less synaptic proteins (arbitrary units) in the hippocampus compared to young rats and the difference is more drastic in low-capacity Runner (LCR) rats than in high- capacity runner (HCR) rats. Western blot analyses of the expression of (A) synapsin (SYN-1) and (B) synaptophysin (SYP) in hippocampal homogenates of HCR and LCR rats at the age of 8 weeks (young) and 10 months (old). The membrane was cut horizontally to separate SYN-1 and SYP according to their molec ular weights, which are shown with the representative blot image. The graphs show the amount of the pro teins normalized to the total protein as analyzed from the stain-free blot. From the stain-free blot only the band of approximately 50 kDa is shown. The boxes and whiskers are the same as in Fig. 1. Mann-Whitney U test. * p ≤0.050, ** p ≤0.010 and *** p ≤0.001.

Article Snippet: Before that, the membranes were blocked for 1 h at RT with OdysseyR Blocking buffer (LI-COR 927–40000, Lincoln, NE, USA) and then incubated overnight at + 4 ◦C with primary antibodies against synaptic markers SYN-1 (ANR-014, Alomone labs, Jerusalem, Israel, 1:1000 dilution) and SYP (ANR-013, Alomone labs, Jerusalem, Israel, 1:1000), glial marker GFAP (ab7260, Abcam, Cambridge, UK, 1:5000 dilution) and microglial marker IBA1 (PA5-27436, ThermoFisher Scientific, Rockford, IL, USA, 1:700 dilution).

Techniques: Western Blot, Expressing, Membrane, Staining, MANN-WHITNEY

Journal: Cancer cell

Article Title: Binary pan-cancer classes with distinct vulnerabilities defined by pro- or anti-cancer YAP/TEAD activity

doi: 10.1016/j.ccell.2021.06.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-Synaptophysin (clone MRQ-40) , Origene , Cat# TA327740.

Techniques: Virus, Plasmid Preparation, Derivative Assay, Recombinant, Electron Microscopy, Staining, SYBR Green Assay, Reverse Transcription, Sample Prep, Microarray, Knockdown, Gene Expression, CRISPR, Software, Flow Cytometry